Forty-four samples of amniotic fluid were studied to evaluate antibacterial activity, of which fifteen samples in midtrimester pregnancy and twenty-nine samples in term were examined by means of colony count method. The antibacterial activity of
amniotic fluid was analyzed using three pathogenic organisms Staphylococcus aureus(S. aureus, ATCC 25923), Escherchia coli(e. coli, ATCC 25922) and Pseudomonas aeruginosa(P. aerusinosa, ATCC 27853). It was found that antibacterial activity of
amnoitic
fluid in term pregnancy was higher than in midtrimester.
Out of 15 cases in midtrimester, 3(20.0%) had only bacteriostatic activity against S. aureus, where as the rest had no antibacterial activity against E. coli and P. aeruginosa. 2 out of 29 cases(6.9%) in term pregnancy had bactericidal activity
against
S. aureus. Bacteriostatic activity against S. auresu, E. coli and P. aeruginosa were 51.7%, 34.5% and 9.1%, respectively with a view to type of strains, becteriostatic samples against P. aeruginosa had same activity against E. coli and S. aureus,
and
stimulatory samples against S. aureus had same activity against E. coli and P. aeruginosa.
In comparison with amniotic fluid, tryptic soy borth (TSB) had greater stimulatory effect and distilled water had same bacteriostatic activity against each strain.
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